| CATHOLIC SOCIAL SERVICES OF THE MIAMI VALLEY | Dayton | — | $30.6M | 3 |
| CEMS OF OHIO INC | Columbus | E62 Health Care | $30.6M | 8 |
| CLARK COUNTY HOSPITAL SERVICES CORPORATION | Dayton | E24 Health Care | $30.6M | 0 |
| EASTWAY CORPORATION | Dayton | F300 Mental Health & Crisis Intervention | $30.4M | 11 |
| StriveTogether Inc | Cincinnati | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $30.3M | 14 |
| NEIGHBORHOOD HEALTH CARE INC | Cleveland | E320 Health Care | $30.3M | 7 |
| PORTSMOUTH HOSPITAL CORPORATION | Portsmouth | E22 Health Care | $30.2M | 9 |
| LAUREL LAKE RETIREMENT COMMUNITY INC | Hudson | A6B Arts, Culture & Humanities | $29.9M | 8 |
| HOMELAND CREDIT UNION INC | Chillicothe | — | $29.7M | 6 |
| Trust for the Post Employment Health Plan for Collectively Bargained Public Employees | Columbus | — | $29.6M | 0 |
| LOWER LIGHTS CHRISTIAN HEALTH CENTER | Columbus | E32 Health Care | $29.5M | 11 |
| LUTHERAN SOCIAL SERVICES OF CENTRAL OHIO | Columbus | — | $29.4M | 7 |
| THE LOZICK FAMILY FOUNDATION | Pepper Pike | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $29.4M | 7 |
| WESTERN RESERVE LAND CONSERVANCY | Moreland Hills | C34 Environment | $29.2M | 0 |
| WRIGHT STATE PHYSICIANS INC | Fairborn | E22Z Health Care | $29.1M | 21 |
| FOUNDATION FOR APPALACHIAN OHIO | Nelsonville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $29M | 2 |
| Barnesville Hospital Association Inc | Barnesville | E220 Health Care | $28.9M | 11 |
| KENDAL AT OBERLIN | Oberlin | P750 Human Services | $28.9M | 9 |
| MENTAL HEALTH SERVICES FOR HOMELESS PERSONS INC | Cleveland | P85Z Human Services | $28.8M | 8 |
| DELTA ZETA NATIONAL HOUSING CORPORATION | Oxford | — | $28.7M | 1 |
| ROCKING HORSE CHILDREN'S HEALTH CENTER | Springfield | E30 Health Care | $28.6M | 9 |
| GREATER COLUMBUS ARTS COUNCIL | Columbus | A260 Arts, Culture & Humanities | $28.6M | 5 |
| Trust For The City of Phoenix Arizona Post Employment Health Plan | Columbus | Y44 Mutual & Membership Benefit | $28.5M | 0 |
| Life Connection of Ohio | Maumee | E65Z Health Care | $28.4M | 10 |
| THE CONVENTION AND VISITORS BUREAU OF GREATER CLEVELAND INC | Cleveland | — | $28.2M | 9 |
| COMMUNITY SUPPORT SERVICES INC | Akron | F320 Mental Health & Crisis Intervention | $28.2M | 7 |
| ReliabilityFirst Corporation | Cleveland | W80 Public & Societal Benefit | $28.2M | 21 |
| KEYBANK FOUNDATION | Cleveland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $28.1M | 0 |
| 1381 RIVER VALLEY CREDIT UNION INC | Miamisburg | — | $28.1M | 8 |
| JEWISH FAMILY SERVICE ASSOCIATION OF CLEVELAND OHIO | Pepper Pike | X30 Religion-Related | $28M | 7 |
| NORTHEAST OHIO NEIGHBORHOOD HEALTH SERVICES INC | Cleveland | — | $28M | 8 |
| INTERNATIONAL UNION OF OPERATING ENGINEERS LOCAL 18 | Cleveland | — | $27.9M | 35 |
| SUNSHINE INC RESIDENTIAL & SUPPORT SERVICES | Maumee | P730 Human Services | $27.9M | 8 |
| BRETHREN RETIREMENT COMMUNITY | Greenville | P750 Human Services | $27.8M | 9 |
| COMMUNITY ACTION AGENCY OF COLUMBIANA COUNTY INC | Lisbon | P20Z Human Services | $27.8M | 7 |
| CARROLL ELECTRIC COOPERATIVE INC | Carrollton | — | $27.7M | 15 |
| DEFIANCE COLLEGE | Defiance | B420 Education | $27.7M | 4 |
| Knox Energy Cooperative Association Inc | Canton | Y22 Mutual & Membership Benefit | $27.6M | 5 |
| DAYTON FOUNDATION DEPOSITORY | Dayton | T31Z Philanthropy, Voluntarism & Grantmaking Foundations | $27.6M | 7 |
| COLLEGE NOW GREATER CLEVELAND INC | Cleveland | B820 Education | $27.6M | 10 |
| GREENE MEMORIAL HOSPITAL | Miamisburg | E220 Health Care | $27.4M | 16 |
| THE GOODWILL INDUSTRIES OF AKRON OHIO INC | Akron | — | $27.2M | 7 |
| MID-OHIO ENERGY COOPERATIVE INC | Kenton | — | $27.1M | 18 |
| Ohio Children's Alliance | Columbus | — | $27.1M | 5 |
| AHF OHIO INC | Dublin | E99 Health Care | $27M | 8 |
| SPECIALIZED ALTERNATIVES FOR FAMILIES & YOUTH OF OHIO INC | Delphos | P320 Human Services | $26.8M | 6 |
| THE TOLEDO MUSEUM OF ART | Toledo | A510 Arts, Culture & Humanities | $26.8M | 8 |
| COLUMBUS HOUSING PARTNERSHIP INC | Columbus | L200 Housing & Shelter | $26.8M | 7 |
| THE SUSANNE GEIER PETERS FOUNDATION C/O RICHARD E GEIER | Loveland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.7M | 0 |
| LIFECARE HOSPICE | Wooster | — | $26.7M | 10 |
