| MISERICORDIA FOUNDATION | Chicago | P110 Human Services | $58M | 2 |
| EASTER SEALS INC | Chicago | — | $58M | 11 |
| Council for Jewish Elderly | Chicago | P750 Human Services | $57.8M | 8 |
| FERRELL HOSPITAL COMMUNITY FOUNDATION | Eldorado | E202 Health Care | $57.8M | 8 |
| Illinois Action for Children | Chicago | — | $57.6M | 8 |
| UCAN | Chicago | P70Z Human Services | $57.6M | 8 |
| Illinois Children's Healthcare | Oak Brook | P12 Human Services | $57M | 1 |
| QUINCY UNIVERSITY CORPORATION | Quincy | B420 Education | $56.5M | 7 |
| LINCOLN PARK ZOOLOGICAL SOCIETY | Chicago | D500 Animal-Related | $56.4M | 8 |
| EMPOWER ILLINOIS | Chicago | B01 Education | $56.1M | 5 |
| Illinois Life & Health Insurance Guaranty Association | Lisle | — | $55.9M | 2 |
| INTERNATIONAL UNION OF OPERATING | Countryside | — | $55.9M | 27 |
| Abbott Fund | Abbott Park | — | $55.6M | 0 |
| PEKIN MEMORIAL HOSPITAL | Urbana | E220 Health Care | $55M | 19 |
| Paul M Angell Family Foundation | Chicago | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $55M | 1 |
| Moose International Inc | Mooseheart | — | $54.6M | 19 |
| Memorial Hospital Association | Carthage | E22 Health Care | $54.5M | 8 |
| THE LATIN SCHOOL OF CHICAGO | Chicago | B200 Education | $54.2M | 9 |
| Greenville College | Greenville | B420 Education | $54.2M | 5 |
| AMERICAN LIBRARY ASSOCIATION | Chicago | B030 Education | $54.1M | 11 |
| ROCHELLE COMMUNITY HOSPITAL ASSOCIATION | Rochelle | E22 Health Care | $53.9M | 9 |
| NUMARK CREDIT UNION | Joliet | W61 Public & Societal Benefit | $53.6M | 10 |
| WADE MCNEILL EXEMPT UNITRUST | Chicago | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $53.5M | 0 |
| CENTERSTONE OF ILLINOIS INC | West Frankfort | F200 Mental Health & Crisis Intervention | $53.2M | 8 |
| ASSOCIATION FOR INDIVIDUAL DEVELOPMENT | Aurora | P82Z Human Services | $52.8M | 7 |
| Carlinville Area Hospital Association | Carlinville | E22 Health Care | $52.4M | 6 |
| ABBOTT LABORATORIES EMPLOYEES | Gurnee | W61 Public & Societal Benefit | $52.2M | 13 |
| FAIRFIELD MEMORIAL HOSPITAL ASSOCIATION | Fairfield | E22Z Health Care | $51.9M | 11 |
| Planned Parenthood of Illinois | Chicago | E420 Health Care | $51.9M | 7 |
| Tri-County Electric Cooperative Inc | Mount Vernon | — | $51.7M | 16 |
| St Joseph's Hospital of the Hospital Sisters of the Third Order of St Franc | Highland | E220 Health Care | $51.7M | 10 |
| AMERICAN VETERINARY MEDICAL ASSOCIATION | Schaumburg | K03 Food, Agriculture & Nutrition | $51.7M | 33 |
| KRAFT HEINZ COMPANY RETIREE HEALTH & | Chicago | Y43 Mutual & Membership Benefit | $51.7M | 1 |
| NORRIS ELECTRIC COOPERATIVE | Newton | — | $51.6M | 18 |
| Chicago Commons Association | Chicago | P200 Human Services | $51.5M | 8 |
| PROSIGHT FINANCIAL ASSOCIATION | Chicago | — | $51.5M | 11 |
| MATHER | Evanston | P19 Human Services | $51.3M | 12 |
| YOUTH CONNECTION CHARTER SCHOOL | Chicago | B20 Education | $51M | 8 |
| Elevate Energy | Chicago | C35 Environment | $50.9M | 6 |
| FRANK E PAYNE AND SEBA B PAYNE | Chicago | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $50.9M | 4 |
| TRILOGY INC | Chicago | — | $50.8M | 7 |
| CORNERSTONE SERVICES INC | Joliet | P800 Human Services | $50.7M | 10 |
| EVANGELICAL RETIREMENT HOMES OF | Schaumburg | P750 Human Services | $50.4M | 10 |
| Francis W Parker School | Chicago | B200 Education | $50.3M | 7 |
| CANTIGNY FOUNDATION | Wheaton | — | $50.1M | 16 |
| WAYNE-WHITE COUNTIES ELECTRIC | Fairfield | — | $50M | 16 |
| PCC COMMUNITY WELLNESS CENTER | Oak Park | E320 Health Care | $50M | 11 |
| SAMARITAN MINISTRIES INTERNATIONAL | Peoria | E80 Health Care | $49.9M | 12 |
| SCHWAB REHABILITATION HOSPITAL AND CARE | Chicago | E220 Health Care | $49.8M | 15 |
| FAMILY GUIDANCE CENTERS INC | Glenview | — | $49.6M | 9 |
