| New York University in Abu Dhabi Corp | New York | B11 Education | $284.1M | 27 |
| Metropolitan Opera Association Inc | New York | A6A0 Arts, Culture & Humanities | $284M | 12 |
| THE NEW YORK COMMUNITY TRUST | New York | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $282.5M | 17 |
| FRIENDS OF THE ISRAEL DEFENSE FORCES | New York | Q123 International, Foreign Affairs & National Security | $282M | 14 |
| NEW YORK QUEENS MEDICINE & SURGERY PC | Flushing | E31 Health Care | $281.9M | 7 |
| THE JEWISH FEDERATIONS OF NORTH AMERICA INC | New York | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $278.7M | 13 |
| THE AYCO CHARITABLE FOUNDATION | Cohoes | T300 Philanthropy, Voluntarism & Grantmaking Foundations | $278M | 0 |
| LABOR-MANAGEMENT HEALTHCARE FUND | Cheektowaga | Y43 Mutual & Membership Benefit | $277.9M | 1 |
| THE EPISCOPAL CHURCH CLERGY AND | New York | — | $277.4M | 1 |
| 1199SEIU GREATER NEW YORK BENEFIT FUND | New York | — | $275.1M | 4 |
| PUBLIC HEALTH SOLUTIONS | New York | H00J Medical Research | $271.2M | 9 |
| St Luke's Cornwall Hospital | Newburgh | E220 Health Care | $268.2M | 14 |
| Teachers College Columbia University | New York | B500 Education | $267.7M | 16 |
| ST CATHERINE OF SIENA MEDICAL CENTER | Smithtown | E20 Health Care | $267.2M | 12 |
| PEF ISRAEL ENDOWMENT FUNDS INC | New York | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $266.7M | 2 |
| CONSUMER REPORTS INC | Yonkers | W900 Public & Societal Benefit | $266.1M | 14 |
| BRYANT & STRATTON COLLEGE INC | Orchard Park | B40 Education | $263.7M | 20 |
| VERA INSTITUTE OF JUSTICE INC | Brooklyn | I20Z Crime & Legal-Related | $263.2M | 14 |
| VISITING NURSE SERVICE OF NEW YORK HOME | New York | E920 Health Care | $263M | 10 |
| CHS PHYSICIAN PARTNERS PC | Roslyn | E31 Health Care | $262.6M | 4 |
| CANTON-POTSDAM HOSPITAL | Potsdam | E22Z Health Care | $262.4M | 14 |
| UNITED JEWISH APPEAL-FEDERATION OF | New York | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $262.2M | 14 |
| LEBANESE AMERICAN UNIVERSITY | New York | B430 Education | $262.2M | 25 |
| Services for the Underserved Inc | New York | — | $261M | 9 |
| WEILL CORNELL IMAGING AT NEWYORK-PRESBYTERIAN | New York | E60 Health Care | $260.1M | 9 |
| PROJECT ORBIS INTERNATIONAL INC | New York | Q300 International, Foreign Affairs & National Security | $259.9M | 11 |
| ST PETER'S HEALTH PARTNERS MEDICAL | Albany | E31 Health Care | $259.5M | 32 |
| MASON TENDERS' DISTRICT COUNCIL | New York | Y43 Mutual & Membership Benefit | $259.2M | 4 |
| BREAKTHROUGH T1D | New York | G800 Diseases, Disorders & Medical Disciplines | $258.1M | 15 |
| COLD SPRING HARBOR LABORATORY | Cold Spring Harbor | U500 Science & Technology | $258M | 12 |
| SCO FAMILY OF SERVICES | Garden City | P20 Human Services | $257.2M | 11 |
| OPEN SOCIETY ACTION FUND INC | New York | — | $257M | 6 |
| SUNSET PARK HEALTH COUNCIL INC | Brooklyn | E80 Health Care | $255.6M | 13 |
| NEW YORK INDEPENDENT SYSTEM OPERATOR INC | Rensselaer | W800 Public & Societal Benefit | $255.3M | 36 |
| THE GOTTESMAN FUND | New York | — | $252.9M | 0 |
| ST CHARLES HOSPITAL CORP | Port Jefferson | E220 Health Care | $248.6M | 11 |
| Nuvance Health Medical Practice PC | Poughkeepsie | E210 Health Care | $247.3M | 23 |
| RESEARCH FOUNDATION | Menands | F050 Mental Health & Crisis Intervention | $246.7M | 13 |
| Municipal Credit Union | New York | — | $246.5M | 26 |
| YOUNG ADULT INSTITUTE INC | New York | P82 Human Services | $241.6M | 8 |
| GLEN COVE HOSPITAL | Westbury | E20 Health Care | $239.8M | 20 |
| THE ROCKEFELLER FOUNDATION | New York | — | $238.1M | 19 |
| THE CENTER FOR NEW YORK CITY | New York | L99 Housing & Shelter | $236.8M | 7 |
| Museum of Modern Art | New York | A510 Arts, Culture & Humanities | $236.7M | 23 |
| NEW PARTNERS INC | New York | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $236.4M | 10 |
| UNITED CEREBRAL PALSY ASSOCIATIONS OF | New York | G200 Diseases, Disorders & Medical Disciplines | $235.4M | 9 |
| LINCOLN CENTER FOR THE PERFORMING ARTS | New York | A610 Arts, Culture & Humanities | $235.1M | 13 |
| CLARKSON UNIVERSITY | Potsdam | B430 Education | $233.4M | 21 |
| NEW YORK HOTEL TRADES COUNCIL & HOTEL | New York | Y20 Mutual & Membership Benefit | $232.9M | 21 |
| LOCAL 94 94A 94B HEALTH & BENEFIT | New York | — | $231.9M | 5 |
