| POUDRE VALLEY REA INC | Fort Collins | — | $163.4M | 17 |
| Ahimsa Foundation | Niwot | D20 Animal-Related | $160.2M | 3 |
| Holy Cross Electric Association Inc | Glenwood Springs | — | $159.4M | 18 |
| Colorado Christian University | Lakewood | B430 Education | $156.6M | 9 |
| YAMPA VALLEY MEDICAL CENTER | Steamboat Springs | E220 Health Care | $147.3M | 13 |
| Mental Health Center of Denver | Denver | F30Z Mental Health & Crisis Intervention | $145.6M | 12 |
| MOUNTAIN VIEW ELECTRIC ASSOCIATION | Limon | — | $144.7M | 18 |
| BOHEMIAN FOUNDATION | Fort Collins | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $139.2M | 2 |
| ROCKY MOUNTAIN UFCW UNIONS & EMPLOYERS HEALTH BENEFIT FUND | Arvada | — | $138.1M | 4 |
| ARC THRIFT STORES INC | Lakewood | P820 Human Services | $137.6M | 12 |
| CREDIT UNIONS CHARTERED IN COLORADO 20 WESTERRA CREDIT UNION | Denver | — | $135.8M | 18 |
| LUTHERAN HOSPITAL ASSOC OF THE SAN LUIS VALLEY | Alamosa | E22 Health Care | $130.4M | 23 |
| Rocky Mountain Health Care Services | Colorado Springs | — | $122.7M | 8 |
| DENVER SCHOOL OF SCIENCE AND TECHNOLOGY INC | Denver | B40 Education | $122.2M | 11 |
| Children's Hospital Colorado Health System | Aurora | E19 Health Care | $121.8M | 3 |
| THE ANSCHUTZ FOUNDATION | Denver | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $120.1M | 8 |
| LA PLATA ELECTRIC ASSOCIATION INC | Durango | — | $118.2M | 25 |
| Colorado Coalition for the Homeless | Denver | P850 Human Services | $116.9M | 16 |
| INTERNATIONAL MEDICAL RELIEF | Loveland | E99 Health Care | $111.6M | 2 |
| SALUD FAMILY HEALTH | Fort Lupton | E20Z Health Care | $111.4M | 11 |
| PEAK VISTA COMMUNITY HEALTH CENTERS | Colorado Springs | E32Z Health Care | $111.4M | 9 |
| WIND CREST INC | Highlands Ranch | P75 Human Services | $104.9M | 8 |
| THE COLORADO HEALTH FOUNDATION | Denver | E21 Health Care | $103.6M | 5 |
| THE COMMUNITY FIRM | Denver | I80 Crime & Legal-Related | $102.2M | 8 |
| SIGNAL BEHAVIORAL HEALTH NETWORK | Greenwood Village | E99Z Health Care | $100.6M | 7 |
| MERCY HOUSING INC | Denver | L200 Housing & Shelter | $100.5M | 26 |
| PREMIER MEMBERS CREDIT UNION | Broomfield | — | $97.3M | 31 |
| COLORADO STATE UNIVERSITY FOUNDATION | Fort Collins | B110 Education | $96.9M | 7 |
| LONGMONT UNITED HOSPITAL | Longmont | E220 Health Care | $95.8M | 13 |
| DENVER DUMB FRIENDS LEAGUE | Denver | D200 Animal-Related | $92.9M | 7 |
| MOUNTAIN WEST CONFERENCE | Colorado Springs | N70 Recreation & Sports | $92.8M | 20 |
| Strive Preparatory Schools | Denver | B29 Education | $92.7M | 12 |
| COLORADO GIVES FOUNDATION | Arvada | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $92.6M | 7 |
| BENEVOLENT HEALTHCARE FOUNDATION | Centennial | G19 Diseases, Disorders & Medical Disciplines | $90.6M | 5 |
| LOWER VALLEY HOSPITAL ASSOCIATION | Fruita | E21Z Health Care | $87.9M | 11 |
| ROCKY MOUNTAIN HUMAN SERVICES | Denver | P700 Human Services | $84.5M | 9 |
| WATERSTONE SUPPORTING ORGANIZATION TRUST 1 | Colorado Springs | S11 Community Improvement & Capacity Building | $82.6M | 2 |
| AURORA COMPREHENSIVE COMMUNITY MENTAL HEALTH CENTER INC | Aurora | F32Z Mental Health & Crisis Intervention | $82.4M | 11 |
| T20 WORLD CUP USA INC | Colorado Springs | N80 Recreation & Sports | $82.3M | 1 |
| JEFFERSON CENTER FOR MENTAL HEALTH | Wheat Ridge | F320 Mental Health & Crisis Intervention | $80.9M | 9 |
| SOUTHWEST HEALTH SYSTEM INC | Cortez | E21Z Health Care | $79.6M | 8 |
| YAMPA VALLEY ELECTRIC ASSOCIATION | Steamboat Springs | — | $78.6M | 23 |
| Delta Montrose Electric Association | Montrose | — | $78.6M | 22 |
| CABLE TELEVISION LABORATORIES INC | Louisville | — | $77.7M | 15 |
| The Ryan Foundation | Greenwood Village | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $77.6M | 2 |
| JOINT RESTORATION FOUNDATION INC | Centennial | E65 Health Care | $77.1M | 8 |
| Children's Hospital Colorado Foundation | Aurora | E110 Health Care | $76.4M | 15 |
| NORTHEAST HEALTH PARTNERS LLC | Greeley | E70 Health Care | $76.3M | 5 |
| COLORADO EARLY COLLEGES | Fort Collins | B25 Education | $75.8M | 6 |
| Summitstone Health Partners | Fort Collins | F30 Mental Health & Crisis Intervention | $75.8M | 10 |
