| LICKING MEMORIAL HOSPITAL | Newark | E220 Health Care | $399.2M | 7 |
| UNIVERSITY OF CINCINNATI PHYSICIANS INC | Cincinnati | — | $383.7M | 32 |
| Community Mercy Health Partners | Springfield | E220 Health Care | $380M | 16 |
| THE CLEVELAND FOUNDATION | Cleveland | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $370.4M | 17 |
| SOUTH CENTRAL POWER COMPANY | Lancaster | — | $368.9M | 23 |
| TRIHEALTH INC | Cincinnati | X00Z Religion-Related | $354.3M | 24 |
| OHIO STATE UNIVERSITY FOUNDATION | Columbus | B11 Education | $348.3M | 0 |
| HEARTLAND HEALTH & WELLNESS FUND | Dayton | E81Z Health Care | $347.8M | 9 |
| PROMEDICA CENTRAL PHYSICIANS | Toledo | E30 Health Care | $338.3M | 12 |
| XAVIER UNIVERSITY | Cincinnati | B430 Education | $332.2M | 12 |
| FAIRFIELD MEDICAL CENTER | Lancaster | E220 Health Care | $324.3M | 13 |
| OHIO CHILD CARE RESOURCE AND REFERRAL | Columbus | — | $307M | 2 |
| EATON CORPORATION VEBA | Brooklyn | — | $306.5M | 0 |
| LIMA MEMORIAL JOINT OPERATING COMPANY | Lima | E210 Health Care | $297.5M | 11 |
| Matthew 25 Ministries Inc | Cincinnati | Q193 International, Foreign Affairs & National Security | $297.3M | 5 |
| Beavercreek Medical Center | Miamisburg | E20 Health Care | $290M | 18 |
| FIRELANDS REGIONAL MEDICAL CENTER | Sandusky | E220 Health Care | $287.3M | 16 |
| GALION COMMUNITY HOSPITAL | Galion | E22Z Health Care | $287.3M | 7 |
| NATIONAL YOUTH ADVOCATE PROGRAM INC | Columbus | P020 Human Services | $279M | 9 |
| Community Blood Center | Dayton | E65 Health Care | $274.8M | 14 |
| PROMEDICA HEALTH SYSTEM INC | Toledo | E210 Health Care | $268.6M | 22 |
| DENISON UNIVERSITY | Granville | B420 Education | $265.4M | 12 |
| THE GREATER CINCINNATI FOUNDATION | Cincinnati | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $263.6M | 9 |
| ATRIUM MEDICAL CENTER | Dayton | E220 Health Care | $262M | 32 |
| OCLC INC | Dublin | B700 Education | $257.7M | 30 |
| TRIHEALTH PHYSICIAN ENTERPRISE CORP | Cincinnati | E60Z Health Care | $257.5M | 17 |
| TRINITY HEALTH SYSTEM GROUP | Steubenville | — | $255.8M | 16 |
| COLUMBUS FOUNDATION | Columbus | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $255.8M | 9 |
| General Electric Credit Union | Cincinnati | — | $251.4M | 17 |
| KNOX COMMUNITY HOSPITAL | Mount Vernon | E220 Health Care | $235.8M | 12 |
| AKRON CHILDREN'S HEALTH COLLABORATIVE | Akron | E02 Health Care | $234.4M | 10 |
| THE CHILDREN'S HOSPITAL | Cincinnati | E110 Health Care | $234.3M | 0 |
| HOLZER HOSPITAL FOUNDATION | Gallipolis | E220 Health Care | $220.8M | 9 |
| Summa Health | Akron | E21 Health Care | $220.6M | 17 |
| KENYON COLLEGE | Gambier | B420 Education | $218.8M | 17 |
| OHIO LABORERS DISCTICT COUNCIL OHIO CONTRACTORS ASSOCIATION | Westerville | — | $215.2M | 0 |
| Cedarville University | Cedarville | B420 Education | $206.7M | 22 |
| THE INDEPENDENT CHARITABLE GIFT FUND | Hudson | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $198.3M | 5 |
| THE FORT HAMILTON HOSPITAL | Miamisburg | E220 Health Care | $189.2M | 17 |
| CareSource Kentucky Co | Dayton | E21 Health Care | $188.3M | 7 |
| MARIETTA HEALTHCARE PHYSICIANS INC | Marietta | E32Z Health Care | $187.9M | 10 |
| THE PROCTER & GAMBLE RETIREE BENEFIT | Cincinnati | — | $186.2M | 0 |
| ASHTABULA COUNTY MEDICAL CENTER | Ashtabula | E22 Health Care | $184.8M | 9 |
| PEDIATRIC ACADEMIC ASSOCIATION INC | Columbus | E24Z Health Care | $182.3M | 9 |
| OHIO OPERATING ENGINEERS | Columbus | — | $182.3M | 2 |
| John Carroll University | University Heights | B430 Education | $171.1M | 16 |
| Christian Aid Ministries | Berlin | X200 Religion-Related | $168.7M | 20 |
| FISHER-TITUS MEDICAL CENTER | Norwalk | E22 Health Care | $167.2M | 11 |
| MERCY MEMORIAL HOSPITAL CORPORATION | Toledo | E220 Health Care | $164.9M | 17 |
| The College of Wooster | Wooster | B420 Education | $163.7M | 16 |
